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345748

Effect of Myrrh Extract on DNA Damage Induced by Mitomycin-C in Human Lymphocytes in vitro

Article

Last updated: 24 Dec 2024

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Subjects

-

Tags

Basic Research

Abstract

Background: Mitomycin-C (MMC) is a well-known antitumor drug and DNA-damaging agent. The genotoxic effects of MMC are basically due to the induction of interstrand cross-links (ICLs) in DNA in addition to other monoadduct lesions. These lesions have the potential to interact with normal cell genetic material and produce secondary tumors in cancer patients. Myrrh is a natural oleo resin and an effective herbal remedy used extensively in traditional medicine. Recently, it has been suggested as a non-mutagenic, antioxidant, and antigenotoxic. Aim: This study aimed to evaluate the effect of myrrh extract (ME) on DNA damageinduced by MMC in normal human isolated peripheral blood lymphocytes (IPBLs)in vitro. Subject, Materials, and Methods: Human IPBLswere cultured and exposed to different concentrations of ME (0.005 and 0.01%) for 3 h before applying MMC (0.1µg/ml).DNA damage was assessed using the alkaline comet assay. Results: Myrrh did not show any DNA-damaging effect. The data revealed a significant reduction in DNA damage in cells pretreated with ME in comparison with those treated with MMC alone. Conclusion: The data strongly suggests that ME may have a protective effect against MMC-induced genotoxicity.
 

DOI

10.21608/scumj.2024.345748

Keywords

genotoxicity, Comet assay, Antigenotoxicity, cross-linking agents

Authors

First Name

Amira E.

Last Name

Mohamed

MiddleName

-

Affiliation

Department of Zoology, Faculty of Science, Suez Canal University, Egypt.

Email

amira_elsaid@science.suez.edu.eg

City

Ismailia

Orcid

0000-0002-7509-5281

First Name

Nour E.

Last Name

Sherif

MiddleName

-

Affiliation

Medical Genetics Unit, Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, Egypt.

Email

-

City

-

Orcid

-

First Name

Eman A.

Last Name

Mohammed

MiddleName

-

Affiliation

Medical Genetics Unit, Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, Egypt.

Email

-

City

-

Orcid

-

First Name

Howyda I.

Last Name

Abdel-Halim

MiddleName

-

Affiliation

Medical Genetics Unit, Department of Histology & Cell Biology, Faculty of Medicine, Suez Canal University, Egypt.

Email

-

City

-

Orcid

-

Volume

27

Article Issue

2

Related Issue

46317

Issue Date

2024-02-01

Receive Date

2024-03-14

Publish Date

2024-02-01

Print ISSN

1110-6999

Online ISSN

2090-2581

Link

https://scumj.journals.ekb.eg/article_345748.html

Detail API

https://scumj.journals.ekb.eg/service?article_code=345748

Order

8

Type

Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

MainTitle

Effect of Myrrh Extract on DNA Damage Induced by Mitomycin-C in Human Lymphocytes in vitro

Details

Type

Article

Created At

24 Dec 2024