Beta
ManageSearchChatDiscover
321377

Association between Type of Gene Polymorphisms of Interleukin 4 and Tumor Necrosis Factor Alpha and Chronicity of Immune Thrombocytopenia in Children

Article

Last updated: 24 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical Research (Medical)

Abstract

Background: Immune thrombocytopenia (ITP) is an acquired autoimmune disease resulting from the production of antiplatelet antibodies. Interleukin 4 (IL4) is an important immunoregulatory cytokine. Also, pediatric ITP patients have excess levels of TNF-α (Tumor Necrosis Factor-alpha). Aim: detection of the frequency of IL4 Variable Number of Tandem Repeat (VNTR intron 3) and TNF-α gene (position -308) polymorphisms among Egyptian ITP children and to correlate these genes with the disease course. Patients and Methods: A case-control study was conducted on 50 Egyptian ITP patients and 50 age and sex-matched healthy controls, we excluded secondary ITP and patients with chronic inflammatory states. Interleukin 4 (VNTR intron 3) and TNF-α gene (position -308) gene polymorphisms were studied using polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP). Results: Most patients with acute ITP have a heterozygous variant of IL4 gene (75%), while 50% of chronic ITP patients have the wild variant of IL4. 43% of acute ITP have a mutant variant of the TNF gene and 50% of chronic ITP patients have a mutant variant of the TNF gene. Conclusion: TNF α gene polymorphism may have a role in ITP susceptibility in children. Also, TNF α and IL4 gene polymorphisms may affect the chronicity of thrombocytopenia.
 

DOI

10.21608/scumj.2023.321377

Keywords

TNF α (-308), IL4 (VNTR intron 3), ITP

Authors

First Name

Mona

Last Name

Ismail

MiddleName

-

Affiliation

Department of Pediatrics, Faculty of Medicine, Port Said University, Port Said, Egypt

Email

mona.mahmoud.ped@gmail.com

City

cairo

Orcid

0000-0002-3028-1734

First Name

Hesham

Last Name

Fathy

MiddleName

-

Affiliation

Department of Pediatrics, Faculty of Medicine, Suez Canal University, Ismailia Egypt

Email

heshamel2002@yahoo.com

City

-

Orcid

-

First Name

Shaimaa

Last Name

Sahmoud

MiddleName

-

Affiliation

Department of Pediatrics, Faculty of Medicine, Suez Canal University, Ismailia Egypt

Email

shaimaa.sahmoud@nhs.net

City

-

Orcid

-

First Name

Amany

Last Name

Hassan

MiddleName

-

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia Egypt

Email

-

City

-

Orcid

-

First Name

Samar M.

Last Name

Elfiky

MiddleName

-

Affiliation

Department of Pediatrics, Faculty of Medicine, Suez Canal University, Ismailia Egypt

Email

-

City

-

Orcid

-

Volume

26

Article Issue

7

Related Issue

42539

Issue Date

2023-07-01

Receive Date

2023-10-14

Publish Date

2023-07-01

Print ISSN

1110-6999

Online ISSN

2090-2581

Link

https://scumj.journals.ekb.eg/article_321377.html

Detail API

https://scumj.journals.ekb.eg/service?article_code=321377

Order

11

Type

Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

MainTitle

Association between Type of Gene Polymorphisms of Interleukin 4 and Tumor Necrosis Factor Alpha and Chronicity of Immune Thrombocytopenia in Children

Details

Type

Article

Created At

24 Dec 2024