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306823

Medium from Umbilical Cord Blood Derived-Hepatoblasts Attenuates Liver Fibrosis in Rats

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Last updated: 24 Dec 2024

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Basic Research

Abstract

Background: The liver is a multifunctional organ in our body. Hepatocyte dysfunction may lead to liver failure. Stem cell (SCs) based therapy is a promising technique for the treatment of liver diseases. Umbilical cord-derived mesenchymal stem cells (UCMSCs) are potentially favorable SCs sources for liver transplantation. Aim: We aimed to investigate the in vivo effectiveness of a medium of transdifferentiated hepatoblasts derived from UCMSCs in regenerating liver fibrosis in a rat model. Methods: UCBMSCs were cultured, and expression of specific CD markers was measured by flow cytometer. They were induced to transdifferentiate under pro-hepatogenic conditions, using mainly hepatic growth factor (HGF) and fibroblastic growth factor. Using RT-PCR, the expression of hepatocyte-specific genes was evaluated. A medium of transdifferentiated hepatoblasts was injected in a rat model of CCL4 liver fibrosis followed by an assessment of macroscopic, microscopic, and serum biochemical markers of liver regeneration. Results: After 21 days in culture, UCMSCs adopted the hepatoblasts phenotype. The expression of alpha-fetoprotein (AFP), albumin (ALB), cytokeratin-18 (CK-18), and glucose-6 phosphatase (G6P) significantly increased in differentiated UCMSCs compared to control cells (P < 0.05). In addition, ALB and AFP levels showed a three-fold increase in the medium of differentiated hepatoblasts. The in vivo effectiveness of the medium of transdifferentiated hepatoblasts derived from UCMSCs was assessed following 4 s.c. injections in CCL4 rats, livers showed reduced fibrosis and enhanced biochemical function compared to both sham and diseased groups. Conclusion: Medium from transdifferentiated hepatoblasts obtained from UCMSCs may be an effective and applicable treatment of liver fibrosis in rats with a potential clinical assessment in human liver diseases.  

DOI

10.21608/scumj.2023.306823

Keywords

UCB-MSCs, differentiation, hepatoblasts, fibrosis, liver regeneration

Authors

First Name

Noha M.

Last Name

Abd El-Fadeal

MiddleName

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Affiliation

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Egypt

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First Name

Nagwan A.

Last Name

Sabek

MiddleName

-

Affiliation

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Egypt

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Orcid

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First Name

Emad F.

Last Name

Ismail

MiddleName

-

Affiliation

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Egypt

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City

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Orcid

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First Name

Taghrid B.

Last Name

El-Abaseri

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-

Affiliation

Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Egypt

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Volume

26

Article Issue

5

Related Issue

42291

Issue Date

2023-05-01

Receive Date

2023-07-07

Publish Date

2023-05-01

Print ISSN

1110-6999

Online ISSN

2090-2581

Link

https://scumj.journals.ekb.eg/article_306823.html

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https://scumj.journals.ekb.eg/service?article_code=306823

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4

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Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

MainTitle

Medium from Umbilical Cord Blood Derived-Hepatoblasts Attenuates Liver Fibrosis in Rats

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Article

Created At

24 Dec 2024