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308017

Comparison of Cell Dynamics and Characterization of Bone Marrow-Derived Stem Cells in Different Passages in Young and Aged Rats

Article

Last updated: 04 Jan 2025

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Basic Research

Abstract

Background: Bone marrow-derived mesenchymal cells (BM-MSCs) isolation and culturing have a great interest in many diseases as cell therapy. The clinical use of cultured stem cells is facing debates and criticism due to the increasing number of unproven cell-based therapies and the appearance of many limitations, including the age of the donor and the number of passages. Aim: Due to the discrepancy in the published data, we investigated changes associated with the first four passages of cultured BM-MSCs from old and young rats, regarding morphological and ultrastructure changes, growth kinetics, and surface cell markers. Methods: Cells were isolated and cultured from young and old Wistar rats' long bones. The morphological and ultrastructural characters, cell surface markers, and growth kinetics of the stem cells were analyzed. Results: BM-MSCs in P1 and 2 showed non-significant differences between young and old cells. Morphologically, cells from P3 and P4 became larger and filled with filaments in both groups. The capacity of the cells to proliferate decreased compared to the prior passages, though, it is still better in the young population. Immunophenotypically, young source of cells showed a significant expression of MSCs surface markers across different passages reaching at least 50% of the cells by P4. While steady expression was experienced in old cells in different passages. Conclusion: the donor age directly influences BM-MSCs morphology, ultrastructure, and proliferation. We assert that cells obtained from P2 and P3 of young rats have the best proliferative index which can be used for future studies.
 

DOI

10.21608/scumj.2023.308017

Keywords

Cell dynamics, Electron Microscope, Flow cytometry, Mesenchymal Stem Cells, Phenotyping

Authors

First Name

Rania A.

Last Name

Galhom

MiddleName

-

Affiliation

Human Anatomy and Embryology Department, Faculty of Medicine, Suez Canal University, Egypt , \Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Mona F.

Last Name

Mansour

MiddleName

-

Affiliation

Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt , \Human Physiology Department, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Noha M.

Last Name

Abd-El Fadeal

MiddleName

-

Affiliation

Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt, \ Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Angie M.

Last Name

Ameen

MiddleName

-

Affiliation

Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt , Human Physiology Department, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Noura R.

Last Name

Abd El-Hamid

MiddleName

-

Affiliation

Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt, Medical Genetics Unit, Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Nahla

Last Name

Imbarak

MiddleName

-

Affiliation

Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt, Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University, Egypt

Email

nahla_mubarak@med.suez.edu.eg

City

-

Orcid

-

First Name

Eman A.

Last Name

Mohammed

MiddleName

-

Affiliation

Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt, Medical Genetics Unit, Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Sally S.

Last Name

Mohammed

MiddleName

-

Affiliation

Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt, Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

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Volume

26

Article Issue

5

Related Issue

42291

Issue Date

2023-05-01

Receive Date

2023-07-15

Publish Date

2023-05-01

Page Start

18

Page End

33

Print ISSN

1110-6999

Online ISSN

2090-2581

Article File

SCUMJ_Volume 26_Issue 5_Pages 18-33.pdf

PDF . 1.4MB

Link

https://scumj.journals.ekb.eg/article_308017.html

Detail API

https://scumj.journals.ekb.eg/service?article_code=308017

Order

3

Type

Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

MainTitle

Comparison of Cell Dynamics and Characterization of Bone Marrow-Derived Stem Cells in Different Passages in Young and Aged Rats

Details

Type

Article

Created At

24 Dec 2024