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306689

Mesenchymal Stem Cells Cultivation and Characterization from the Bone Marrow of Adult Male Albino Rats

Article

Last updated: 24 Dec 2024

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Basic Research

Abstract

Background: Stem cells are self-renewing, undifferentiated cells that can be differentiated into functional cells. Mesenchymal stem cells (MSCs) are a subpopulation of mesodermal stem cells population that can be found in numerous living tissues along with bone marrow. Numerous studies have begun to reveal the characteristics of MSCs, which go far beyond forming connective tissue and their simple derivation from various tissues, making them attractive for research. Aim: The present study aimed to isolate, culture, and characterize the MSCs from the bone marrow of long bones of adult male albino rats. Materials and Methods: both femoral bones and tibia were obtained from adult male albino rats. Their bone marrow was washed using complete media formed of DMEM + 10% FPS+ 1% Pen/strept. The mixture was seeded in the culture flask and incubated at 370 humidified atmosphere containing a 5% co2 incubator. The culture flasks were examined under a light microscope to monitor the growth of cells. The cells were then harvested and subcultured using the same technique. Subcultured cells were identified using CD 34 & 44 surface markers. Results: the subcultured MSCs appeared as a sheet of adherent cells having a polygonal appearance with prominent nucleolus & a well-defined cell process. Most of the cells showed positive expression of the CD44 surface marker and negative expression of CD 34. Conclusions: cells that were isolated and cultured from the bone marrow of adult male albino rats had the morphological & immunophenotyping characteristics of MSCs.
 

DOI

10.21608/scumj.2023.306689

Keywords

Stem cell, Bone marrow, culture

Authors

First Name

Nahla S.

Last Name

Saad

MiddleName

-

Affiliation

Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Egypt

Email

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City

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Orcid

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First Name

Ghada S.

Last Name

Abdelkader

MiddleName

-

Affiliation

Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Egypt

Email

dranatomy103@yahoo.com

City

-

Orcid

-

First Name

Noha A.

Last Name

Salem

MiddleName

-

Affiliation

Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Mona H.

Last Name

Ali

MiddleName

-

Affiliation

Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Magdy M.O.

Last Name

El-Fark

MiddleName

-

Affiliation

Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

Volume

26

Article Issue

5

Related Issue

42291

Issue Date

2023-05-01

Receive Date

2023-07-06

Publish Date

2023-05-01

Page Start

12

Page End

17

Print ISSN

1110-6999

Online ISSN

2090-2581

Article File

SCUMJ_Volume 26_Issue 5_Pages 12-17.pdf

PDF . 760.4kB

Link

https://scumj.journals.ekb.eg/article_306689.html

Detail API

https://scumj.journals.ekb.eg/service?article_code=306689

Order

2

Type

Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

MainTitle

Mesenchymal Stem Cells Cultivation and Characterization from the Bone Marrow of Adult Male Albino Rats

Details

Type

Article

Created At

24 Dec 2024