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283960

Role of Heme Oxygenase (HO)-1 Enzyme in the Protective and Therapeutic Effect of Omega 3 Fatty Acids on Cisplatin-induced Hepatic and Renal Toxicity in Rats

Article

Last updated: 04 Jan 2025

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Basic Research

Abstract

Background: Cisplatin is frequently used as an anticancer medication. Nephrotoxicity and hepatotoxicity are triggered by its usage, causing patients to limit their long-term therapy. Aim: To investigate the underlying mechanism of omega-3 (fatty acids) effect on hepatorenal toxicity induced by cisplatin in rats and to detect whether it has a protective or therapeutic effect or both and the role of HO-1 enzyme in both effects. Materials and Methods: 40 male rats were divided into four equal groups: Control group: received i.p. saline+ corn oil orally, Cisplatin Group: received i.p. CP (12 mg/kg)+ corn oil orally, Cisplatin+ ω-3 pretreatment group: received i.p. CP following 10 days of ω-3 pretreatment in dose {(270 mg/kg) EPA, (180 mg/kg)} and Cisplatin+ ω-3 post-treatment group: received i.p. CP followed by 10 days of ω-3 post-treatment in dose as the previous group. Liver and kidney function, serum (HO-1), serum& tissue (TNF-α, IL-10), tissue (NFkB, GSH, MDA), and NrF2 gene expressions were measured. Results: Cisplatin-induced marked hepatorenal failure; detected by elevation of serum: AST, ALT, creatinine, and urea. Also, serum& tissue (TNF-α, IL-10), and tissue (NFkB, GSH, MDA) were significantly changed with no change in NrF2 gene expressions as compared to the control. On other hand, pre or post-ω-3 intake significantly corrected the changed markers. Liver and renal histopathological and immunohistochemical changes confirmed the biochemical results in all groups. Conclusion: Cisplatin treatment impairs liver and kidney function, while ω-3 supplementation could avoid this toxicity, with the protective response appearing to be more beneficial than the therapeutic effect.
 

DOI

10.21608/scumj.2023.283960

Keywords

omega-3, Cisplatin, Heme oxygenase-1, hepatic toxicity and renal toxicity

Authors

First Name

Marwa

Last Name

Awad

MiddleName

M.

Affiliation

Physiology Department, Faculty of Medicine, Tanta University, Egypt.

Email

marwa.saleh@med.tanta.edu.eg

City

-

Orcid

-

First Name

Rania

Last Name

Abd-Ellatif

MiddleName

N.

Affiliation

Biochemistry Department, Faculty of Medicine, Tanta University, Egypt.

Email

rania.emam@med.tanta.edu.eg

City

-

Orcid

-

First Name

Sarah

Last Name

Ibrahim

MiddleName

-

Affiliation

Anatomy Department, Faculty of Medicine, Tanta University, Egypt.

Email

sara.ibrahim@med.tanta.edu.eg

City

-

Orcid

-

First Name

Maaly

Last Name

Abd Elmaaboud

MiddleName

A.

Affiliation

Pharmacology Department, Faculty of Medicine, Tanta University, Egypt

Email

maali_abdelmaboud@med.tanta.edu.eg

City

-

Orcid

-

First Name

Rehab

Last Name

El-Shaer

MiddleName

A.A.

Affiliation

Physiology Department, Faculty of Medicine, Tanta University, Egypt.

Email

-

City

-

Orcid

-

Volume

26

Article Issue

1

Related Issue

39420

Issue Date

2023-01-01

Receive Date

2023-02-02

Publish Date

2023-01-01

Page Start

17

Page End

39

Print ISSN

1110-6999

Online ISSN

2090-2581

Article File

SCUMJ_Volume 26_Issue 1_Pages 17-39.pdf

PDF . 2.7MB

Link

https://scumj.journals.ekb.eg/article_283960.html

Detail API

https://scumj.journals.ekb.eg/service?article_code=283960

Order

3

Type

Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

MainTitle

Role of Heme Oxygenase (HO)-1 Enzyme in the Protective and Therapeutic Effect of Omega 3 Fatty Acids on Cisplatin-induced Hepatic and Renal Toxicity in Rats

Details

Type

Article

Created At

24 Dec 2024