Due to parenteral exposure, injection drug users are very susceptible to infection with the HIV virus, the hepatitis B virus, and the hepatitis C virus. It is commonly known that injecting drug users have a high risk of viral infections such as hepatitis B virus (HBV), and hepatitis C virus (HCV), due to parenteral exposure. The Present study aims to evaluate the incidence of Co-infection of hepatitis B and C viruses among injection drug users. In the Present study we investigated one hundred persons which obtain drug substances aiming to evaluate the epidemiology of Co-infection with HCV and HBV among infection drug users. The present study includes serological, molecular, Hematological, and biochemical techniques. for the patients and control samples. The results indicated that the virus titer was high in the patients used Heroin and Tramadol drugs substances more than the other drug substance and the control. We concluded from this that the other drugs substances (THC and Synthetic Cannabinoids') had an antiviral effect on the virus's replication, in addition of the host immunity. The study concludes that the risk of liver and kidney damage is due to infectious hepatitis in injecting drug users. Measures should be implemented to combat the spread of drug abuse.