A comprehensive investigation into the green synthesis of silver nanoparticles (AgNPs) using botanical reducing agents derived from clove buds (CB), green tea leaves (GT), and orange peels (OP). The research highlights the significant influence of these botanical sources on the yield, stability, and nanoscale characteristics of the biosynthesized AgNPs. Notably, OP emerged as the most efficient reducing agent that produce AgNPs with the highest chemical yield (81%) and highest suspension stability (zeta potential of -72mV). While CB demonstrated superior biocompatibility and lower cytotoxicity across multiple cell lines. These findings underscore the potential of CB as a promising candidate for the synthesis of AgNPs, with implications for diverse biomedical applications. This research paves the way for future studies aimed at harnessing the versatility of botanical reducing agents, particularly CB, in the green synthesis of AgNPs and their potential applications in the biomedical and environmental fields. Further exploration of CB's potential beyond antimicrobial and cytotoxicity assessments is recommended. This study thus provides a significant contribution to the field of green nanotechnology, emphasizing the importance of selecting appropriate botanical reducing agents for the optimized synthesis of AgNPs.