White sapote is a fruit that has many biological effects on human health including antidiabetic, antidepressant, antioxidant, anticancer and immunomodulatory activities. It contains various dietary constituents including phenolics, carotenoids, polysaccharides and vitamins. The in vitro culture system should provide the best way to develop and produce the valuable biological compounds of White sapote. To establish a protocol for callus induction of White sapote and compare its antioxidant activity in callus culture from different explants, plant growth regulators (also known as PGRs) were applied in several treatments. Various explants were grown on MS media. These media contained PGRs in five different treatments, including a combination of Benzyl Adenine (BA) with Naphthalene Acetic Acid (NAA). Most of the evaluated explant types produced 100% callus induction. (nodal segments, shoot tips, and root segments) on a medium provided 0.5 mg/L BA with 2 mg/L NAA; however, leaf segments didn't produce any callus. Under the medium including 2 mg/L NAA along with 0.5 mg/L BA, shoot-tip explants produced the largest callus fresh mass (1.05 gm). The amounts of antioxidant activity were estimated and the noticeable difference was acquired from various PGR treatments in the callus. These findings suggest that specific PGRs increase the accumulation of different secondary metabolism products and have influence on the production of callus from white sapote. Thus, the culture protocol detailed in this study may provide a novel method for micropropagation of this plant from callus and for secondary metabolic products from white sapote.