Two field experiments investigated the impact of nitrogen fertilizer on bioethanol production from various sweet sorghum varieties in Egypt. The study, conducted across two seasons, analyzed the effects of fertilizer rates (60, 90, and 120 kg N/fed) on stalk length, sucrose content, stripped stalk yield, juice yield, and ethanol yield. Significant variations were observed in most studied traits across both seasons, except juice extraction percentage, which only differed significantly in the second season. Sweet sorghum plants receiving the highest nitrogen application (120 kg N/fed) demonstrated the most desirable outcomes for most traits. Conversely, the lowest fertilizer application (60 kg N/fed) resulted in the least favorable outcomes across both seasons. Additionally, significant differences were noted between the sweet sorghum varieties themselves. SS 301-1 and MN 1500 varieties consistently achieved the highest values for most studied traits, except for juice extraction percentage in the second season, where Rex and Brands varieties excelled. In conclusion, the study suggests that applying 120 kg N/fed of nitrogen fertilizer to SS 301-1 sweet sorghum variety can potentially enhance sucrose content and bioethanol production within the specific soil conditions of the Egyptian experiment.