This work included the green synthesis of silver nanoparticles (AgNPs) utilizing an aqueous extract of Syzygium cumin L. leaves. The aqueous extract was analyzed qualitatively and quantitatively for phytochemical content. The morphological features, optical characteristics, and surface properties of synthesized AgNPs were examined using X-ray diffraction (XRD), transmission electron microscopy (TEM), ultraviolet-visible (UV-Vis) absorption spectroscopy, and Fourier transform infrared spectroscopy (FTIR). the analysis revealed that the concentrations of flavonoids, tannins, phenolics, and flavonols were 194.77±0.55 µg RT /g, 46.02±0.53 µg TAE/g, 107.25.92±0.5 µg GAE/g, and 32.6±0.67 µg RT /g, respectively. The plant extract demonstrated antioxidant activity, with an IC50 value of 13.54±1.1 µg/mL-1, attributed to its high content of flavonoid and phenolic compounds, suggesting its potential as a natural source of antioxidants. The TEM study demonstrated that the bio-synthesized AgNPs exhibited a remarkable level of homogeneity in relation to their surface morphology. The mean diameter of the particles was around ~55 nanometers. The green synthesized Ag-NPs showed notable antibacterial activity against several bacterial species. The diameter of the inhibition zones ranged from 26 to 28 mm against six different bacterial isolates, namely S. aureus, S. haemolyticus, E. faecalis, A. baumannii, K. pneumoniae, and E. coli. The AgNPs had a dose-dependent impact on the stabilization of the red blood cell in human (HRBC) membrane. This led to a varying degree of prevention of hemolysis, ranging from 77.6 ± 1.1% to 95.2 ± 1.4%. The present work introduces a straight forward and economically viable approach for synthesizing AgNPs using environmentally friendly.