Two field trials were carried out over two summer seasons in 2021 and 2022 in soybean crop (Glycin max L.) Giza 111 was planted at the experimental farm at Itay EL-Baroud-Agricultural Research Station, Itay EL-Baroud, Beherah Governorate, Egypt to evaluate the effect of four pre-emergence herbicides as acetochlor 84% EC (Harness), butralin 96% EC (Amex), pendimethalin 45.5%CS (Stomp Extra) and s-metolachlor 96 % EC (Gardo ) as well as hand hoeing This was done (twice at 21 and 35 days after sowing (DAS) at 60 DAS, the unweeded control was used to weed control biomass (gmm-2) of broad-leaved, grass, and total weeds in soybean fields. Results showed in both seasons that all weed management methods considerably reduced weed parameters while increasing yield components. It resulted with a significantly substantial increase in seed soybean yield (Kg fed-1). During two seasons, butralin (97.82 and 96.18%) and acetochlor (94.94 and 94.23%) were found to be effective. The maximum effect on soybean leaf biochemical after 30 days of seeding was obtained as (chlorophyll a, chlorophyll b, total chlorophyll (mgg-1 fresh weight), total carbohydrate (%), protein content (%), and total phenolic content (%) with all the tested herbicides.