Twenty dry, nonpregnant ossimi ewes were used to examine how several physiological parameters were affected by a oneday exposure to heat stress. Animals were split equally into two groups, with each group consisting of two experimental ewes, each of which was aged 3–4 years and weighed 35–40 kg (NRC 1988). The trial began on July 15, 2019, and ended on December 1. On the first day of the experiment, the first group was subjected to heat stress for a whole day. The control group was kept in the shadow until the experiment's conclusion. The levels of creatinine, haemoglobin (HB), glutathione peroxidase, total antioxidant capacity, glutathiones transferase, (ALT & AST), and body temperature were measured. The findings demonstrated that although the control group underwent no changes, those exposed to just one day of summertime direct heat experienced an increase in oxidative stress enzymes. That also happened when people were exposed to direct cold for one day during the winter, which increased their levels of oxidative enzymes in comparison to the control group. This is referring to exposure to direct heat or direct cold that affects an animal's immunity and causes an increase in enzymes as a defence mechanism the animal uses to withstand pressure.