Hepatitis virus C (HCV) is a serious public health issue worldwide. HCV is a cause of liver fibrosis, which may progress to cirrhosis or hepatocellular cancer. To assess the current study goal, we evaluated parameters including liver function, tumor marker, hematological, renal functions, lipid profile, and blood sugar levels in two groups (healthy controls and hepatitis C virus patients) to evaluate the current research's aim. Based on the results of seven clinical tests, we observed insignificant changes in Cr, TSH, Alb, GCT, Alk.PH, Bill-total, Bill-direct, WBC, AFB, Cholesterol, and TG between the control and patient groups, there were significant differences for AST, ALT, Hb, platelets, INR, PT, PC percent, LDL, and FBS. The Hepatitis C virus profoundly affects blood parameters and body physiology in HCV patients. In the present study, 15 patients were sampled, and their HCV NS5B regions were directly sequenced and analyzed phylogenetically to learn more about HCV molecular epidemiology. According to the molecular epidemiology study, those who were treated with Sofosbuvir were found to have a high occurrence of certain genotypes.