Beta
341661

Phytochemistry and Biodiversity of Endophytic Fungal Metabolites Isolated from Medicinal Plants

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

Biotechnology

Abstract

It is critical to study endophytic fungi because they boost plant production which benefits both people and animals and meets nutritional needs. Four medicinal plants "Datura sramonium, Echinacea purpura, Salvia rosmarinus and Thymus vilgrus" were used to isolate twelve different fungal species. Preliminary light microscope identification showed that these species belonged to three genera Asprigillus species, Alternaria species, and Fusarium species. Antimicrobial activity was done for the intracellular (Mat) and extracellular extracts of all isolated fungi and showed that the extracellular extracts had higher activity than the intracellular (Mat) extracts. "Candida albicans" was the most sensitive fungal species, creating a disincentive zone (11±0.4 mm) with an (E2) fungal isolate, and "Pseudomonas aurogonosa" was the most sensitive bacterial species, producing a disincentive zone (26±0.5 mm) with an (E2) endophytic fungal isolate. Additionally, utilising (1, 1) diphenyl-2-picrylhydrazyl (DPPH) scavenging activity to assess the antioxidant activities for the twelve fungal isolates, it has been found that (T1) and (T3) isolated fungi have the highest antioxidant activities, with IC50 values of 36.8 and 36.8 µg/ml, successively. Alkaloids, flavonoids, anthocyanins, tannins, and anthraquinones were found in the extracellular and intracellular (Mat) fungal extracts, which were separated using thin layer chromatography (TLC), demonstrating that the extracellular cell extracts have a higher concentration of these phytochemical groups than the intracellular cells. Additionally, the polymerase chain reaction (PCR) was used to completely identify the two most active isolated fungi (R2 and T3), identifying both isolates as Alternaria Alternata.

DOI

10.21608/ajar.2024.224023.1198

Keywords

medicinal plants, Fungal endophyes, Antimicrobial, antioxidant, TLC

Authors

First Name

Said

Last Name

Olama

MiddleName

M.

Affiliation

Department of Microbiology, Faculty of Science, Al Azhar University, Cairo, Egypt.

Email

saidolama9@gmail.com

City

Benha

Orcid

-

First Name

Reda

Last Name

Sheata

MiddleName

-

Affiliation

The Regional Center for Mycology and Biotechnology, Al Azhar University, Cairo, Egypt.

Email

reda.sheata@gmail.com

City

Cairo

Orcid

-

First Name

Hussien

Last Name

El Shiekh

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Science, Al Azhar University, Cairo, Egypt.

Email

hseigekuyf@yahoo.com

City

Cairo

Orcid

-

First Name

Ahmed

Last Name

Younis

MiddleName

M.

Affiliation

Department of Microbiology, Faculty of Science, Al Azhar University, Cairo, Egypt.

Email

ahmedyounis@gmail.com

City

Cairo

Orcid

-

Volume

48

Article Issue

2

Related Issue

46166

Issue Date

2023-12-01

Receive Date

2023-07-19

Publish Date

2023-12-01

Page Start

42

Page End

58

Print ISSN

1110-1563

Online ISSN

2786-0051

Link

https://ajar.journals.ekb.eg/article_341661.html

Detail API

https://ajar.journals.ekb.eg/service?article_code=341661

Order

341,661

Type

Original Article

Type Code

929

Publication Type

Journal

Publication Title

Al-Azhar Journal of Agricultural Research

Publication Link

https://ajar.journals.ekb.eg/

MainTitle

Phytochemistry and Biodiversity of Endophytic Fungal Metabolites Isolated from Medicinal Plants

Details

Type

Article

Created At

24 Dec 2024