Infection with the Hepatitis C virus (HCV) is a severe public health concern worldwide. HCV is a prevalent cause of liver fibrosis, which can progress to cirrhosis or hepatocellular cancer. To assess the research objective, we evaluated parameters including liver functions, liver tumor markers, hematological, renal functions, lipid profile, and blood sugar in two groups: the healthy control group with the group of hepatitis C virus patients. In the presented study, albumin (ALB), alkaline phosphatase (ALK PH), and Bilirubin total (BIL T) were compared, and there were no remarkable variations between the control and patient groups. On the other hand, -γglutamyl transaminase (GGT), aspartate transaminase (AST), alanine transaminase (ALT), and bilirubin direct (Bill D) showed noticeable differences between the two groups. Regarding the lipid profile, including cholesterol (CHO), triglyceride (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), There were no statistically significant variations between the control and patient communities. Therefore, data analysis for HCV biochemical markers and physiological activities was critical for understanding HCV's epidemiology and treatment methods in Egyptian chronic patients.