Investigating bovine and ovine mycotic mastitis was the study's goal. A total of 250 milk samples were collected from cows, ewes and does suffering from mastitis, not responding for antibiotic therapy, to determine the prevalence of fungal pathogens associated with mastitis. For the isolated yeasts, antimicrobial susceptibility testing was done through disc diffusion method, and biofilm formation was evaluated phenotypically by cultivation on Congo Red Agar (CRA) medium. Also, MDR1, CDR and ERG11 genes were detected by PCR. Out of the collected milk samples, 53 samples (21.2%) were positive for the presence of fungal pathogens. Out of them, 54 fungal pathogens were recovered, including 49 Candida species, 2 A. niger, in addition to one of A. fumigatus, A. flavus and Penicillium species. The yeast isolates included 18 C. guilliermondii, 16 C. parapsilosis, 7 C. tropicalis, 4 C. albicans, and 4 C. kefyr. Antimicrobial susceptibility testing of Candida isolates showed that all isolates were resistant to nystatin. Regarding to fluconazole, 25 isolates were sensitive, 2 isolates were intermediately sensitive and 22 isolates were resistant. Nine yeast isolates were biofilm former. All tested isolates harbored MDR1 gene, 4 isolates contained CDR gene and only 1 isolate was positive for ERG11 gene.