This study was focused on the prevalence of E. coli in mastitis and endometritis  of sheep and goats in Beni-Suef government with a total number of 238 samples  as follow;  122 of native breed lactating ewes (n=90) and does (n=32) from private farms suffered from mastitis  and a total of 116 of native breed of ewes (n=83) and she-goats (n=33) from endometritis cases in which137 isolates of Escherichia coli (E. coli) categorized as 68 isolates were isolated from mastitis and 69 isolates from endometritis with a prevalence rate of 55.7 % and 59.5 %, respectively. Antibiogram for E. coli isolates recovered from ovine and caprine mastitic cases showed resistance to amoxicillin-clavulanic acid (67.65%), cephalexin (64.71 %), amoxicillin (58.82%), cefotaxime (57.42 %), ceftriaxone (54.42 %) and gentamicin (44.11%). On the contrary, the recovered E. coli isolates from endometritis of ewes and does showed resistance to amoxicillin-clavulanic acid (69.57%), cefotaxime (68.11 %), amoxicillin (66.66 %), cephalexin (65.22%), ceftriaxone (62.32 %), colistin (46.38%) and gentamicin (44.93%). Sixty E. coli isolates (27 isolates from ovine mastitis milk samples and 33 isolates from endometritis samples were inoculated onto Yeast extract-casamino acids Congo red agar (YESCA CRA) revealed that 39 of E. coli isolates showed positive biofilm formation. Multidrug resistance was detected in 70 E. coli isolates out of 137 isolates (51.09%) (31 E. coli isolates from mastitis milk samples and 39 isolates from endometritis samples). The prevalence of virulence and resistance genes of E. coli as fimH, iutA, qnrA and bla_TEM was 100%, 25%, 50% and 100%, respectively. This work was designed for detection of virulence and antibiotic resistant E. coli isolates from mastitis and endometritis cases in sheep and goats.