N-(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-Pyrazol-4-yl)carbonohydrazonoyl dicyanide(LB) is operated to produce and describe the novel Co(II) complex (CB). The structural compositions of the new compounds were clarified through the utilization of analytical and spectroscopic techniques. This study examines the effects of gamma radiation on the Co(II) complex of the ligand and its biological activity (B and A before and after irradiation). Theoretical measurements supported the proposed generic formula for the [CoLCl2 (H2O)2] complex as well as the results of thermal analysis, FTIR, molar conductivity and elemental analysis, the complex produced with a molar ratio of 1:1 M:L. The antibacterial activities of the chemical were assessed using ampicillin, gentamicin as standards, and the following sequence of activities was observed: (CA) presented the highest activity, followed by CA > CB > LA > LB. The molecular docking process's objective study was to investigate the binding between the complex with Penicillin-binding protein PBP, which is responsible for supporting microbial inhibitory growth.