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357025

Polymorphic Variations of ACE2 and TMPRSS2 Genes in Egyptian COVID-19 Patients: Correlation with Disease Severity and Sex-Related Gene Variations

Article

Last updated: 24 Dec 2024

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Abstract

Abstract:
This research pointed to accessing the polymorphic variations of the ACE2 and TMPRSS2 genes in Egyptian COVID-19 patients and their potential correlation with disease severity. The study involved a total of ninety-six participants, with forty-eight experiencing severe symptoms and an equal number exhibiting mild symptoms. Genotyping of single nucleotide polymorphisms (SNPs) in ACE2 and TMPRSS2 was performed using PCR-RFLP analysis.
The median age of individuals with mild cases was 35 years (range: 30-41 years), while for severe cases, it was 65 years (range: 58 to 72 years). The male-to-female ratio in the entire group was 47.9% to 52.1%. All participants tested positive for COVID-19 based on nasal swab qPCR tests.
Regarding genotypic distribution, the TT genotype of TMPRSS2 rs12329760 was found to be statistically more prevalent in the severe group compared to the mild group. However, no significant differences were observed in the distribution of ACE2 rs2285666 genotypes or alleles between the two groups. Concerning gene variations associated with sex in severe cases, males displayed a higher occurrence of the AA genotype, while females exhibited a higher prevalence of the GA genotype.

DOI

10.21608/rjab.2024.250880.1046

Keywords

COVID-19, ACE2, TMPRSS2, single nucleotide polymorphisms, PCR-RFLP

Authors

First Name

Faten

Last Name

Saad

MiddleName

M.

Affiliation

Molecular diagnostics and therapeutics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

Email

faten.saad@gebri.usc.edu.eg

City

-

Orcid

-

First Name

Ahmed

Last Name

Daif

MiddleName

-

Affiliation

Molecular diagnostics and therapeutics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

Email

ahmed.daif@gebri.usc.edu.eg

City

-

Orcid

-

First Name

Moustafa

Last Name

Sakr

MiddleName

-

Affiliation

Molecular diagnostics and therapeutics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

Email

mostafa.sakr@gebri.usc.edu.eg

City

Sadat City

Orcid

0000-0002-5873-8468

First Name

Hisham

Last Name

Ismail

MiddleName

-

Affiliation

Molecular diagnostics and therapeutics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

Email

hisham.ismail@gebri.usc.edu.eg

City

-

Orcid

-

Volume

10

Article Issue

1

Related Issue

48011

Issue Date

2024-06-01

Receive Date

2023-11-23

Publish Date

2024-06-01

Page Start

15

Page End

26

Print ISSN

2356-9433

Online ISSN

2682-3470

Link

https://rjab.journals.ekb.eg/article_357025.html

Detail API

https://rjab.journals.ekb.eg/service?article_code=357025

Order

2

Type

Original Article

Type Code

890

Publication Type

Journal

Publication Title

Research Journal of Applied Biotechnology

Publication Link

https://rjab.journals.ekb.eg/

MainTitle

Polymorphic Variations of ACE2 and TMPRSS2 Genes in Egyptian COVID-19 Patients: Correlation with Disease Severity and Sex-Related Gene Variations

Details

Type

Article

Created At

24 Dec 2024