Beta
312639

An innovative extracellular polysaccharide produced by Egyptian Marine Bacillus licheniformis, and its applications as antibacterial agent.

Article

Last updated: 24 Dec 2024

Subjects

-

Tags

-

Abstract

Twenty-two marine bacterial isolates were collected from the Eastern Harbor (29885 E longitude and 31205 N latitude) located at Alexandria, Egypt selected to produce exopolysaccharides, using the Shene extraction method in 2008. The most potent marine bacterium was identified as Bacillus licheniformis SMRH1, accession number ON777836 by using 16s rRNA as a molecular identification technique. According to the chemical analysis, the obtained polysaccharide content is estimated by using Fourier Transform Infrared spectra FTIR spectrum of the extracted exopolysaccharide from Bacillus licheniformis which showed that it contains νOH at 3420cm-1, νC-H aliphatic at 2952cm-1, νC=O in range 1660-1630cm-1 and Nuclear magnetic resonance spectra NMR showed signals δ'S ppm at 1.22 (s, -CH3), 2.07 (s, -CH2 chain) and 2.35(m, -CH2) and Scanning electron microscope SEM which confirmed the amorphus shaped uneven texture polysaccharide at higher magnification of ×5000. The exopolysaccharide that was generated has antibacterial activity by forming a free hollow zone around it against some gram positive and gram-negative bacteria according to disc diffusion method. The innovatively produced exopolysaccharide also has anticoagulant activity. A recent study proved that the generated exopolysaccharide induced β-hemolysis. The high yield of exopolysaccharide was “0.7 g/l" by using different culture media such as nutrient broth, peptone water, and king ̓s B broth.

DOI

10.21608/rjab.2023.198462.1027

Keywords

Marine bacteria, Bacillus licheniformis, exopolysaccharide, Chemical Characterization, Antibacterial

Authors

First Name

Sokara

Last Name

Ali

MiddleName

-

Affiliation

Department of Microbial Biotechnology,Genetic engineering and biotechnology research institute,Sadat city university,Egypt

Email

sokaraabdelaziz@yahoo.com

City

Giza

Orcid

0000-0002-3116-2673

First Name

Mohamed

Last Name

Amer

MiddleName

-

Affiliation

Microbiology Laboratory, Environment Division, National Institute of Oceanography and Fisheries, Alexandria, Egypt.

Email

mohammed_saleh74@yahoo.com

City

-

Orcid

-

First Name

Hanafy

Last Name

Hamza

MiddleName

Ahmed

Affiliation

Department of Microbial Biotechnology,Genetic engineering and biotechnology research institute,Sadat city university,Egypt

Email

hanafi.hamza@gebri.usc.edu.eg

City

-

Orcid

-

First Name

Rateb

Last Name

Abbas

MiddleName

Nabil

Affiliation

Department of Microbial Biotechnology,Genetic engineering and biotechnology research institute,Sadat city university,Egypt

Email

rateb.youssef@gebri.usc.edu.eg

City

SADAT

Orcid

-

Volume

9

Article Issue

1

Related Issue

39715

Issue Date

2023-06-01

Receive Date

2023-03-07

Publish Date

2023-07-30

Page Start

28

Page End

37

Print ISSN

2356-9433

Online ISSN

2682-3470

Link

https://rjab.journals.ekb.eg/article_312639.html

Detail API

https://rjab.journals.ekb.eg/service?article_code=312639

Order

3

Type

Short Communication

Type Code

2,525

Publication Type

Journal

Publication Title

Research Journal of Applied Biotechnology

Publication Link

https://rjab.journals.ekb.eg/

MainTitle

An innovative extracellular polysaccharide produced by Egyptian Marine Bacillus licheniformis, and its applications as antibacterial agent.

Details

Type

Article

Created At

24 Dec 2024