New complexes for Mn²⁺ and Cu²⁺ were prepared and characterized from ligand, 2-(phenylglycyl)-N-(p-tolyl)hydrazine-1-carbo thioamide (H2L) and binuclear Cu2+ complex (B3) of hydrazone derivatives . The structural compositions of the novel chelates were clarified using spectral, molar conductivity, FTIR, UV-Visible spectra,TGA, XRD and analytical techniques. The outcomes supported the ligand's neutral bidentate or monobasic tridentate behaviour. Coordination bonding has occurred via carbonyl oxygen (C=O) and N(2)H or (C-S) groups in complex (B1). The synthesized mononuclear complexes B1and B2 displayed an octahedral  , square  planar form . While B3 complex is binuclear . Also, the Cu²⁺complex (B2) has a stronger antibacterial and anticancer activity than the Mn2+complex and the ligand when tested against different bacterial species and the HepG2 cell line. Respectively; The binding ability of ligand and Cu²⁺ complex (B2) with CT-DNA was aimed  to for being intercelation or alternative strategy. The constant of  intrinsic binding Kb was estimated .