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371767

Enhancing Callus Initiation and Salinity Tolerance in Egyptian GZ650 Maize (Zea mays L.) Using Zinc Oxide Nanoparticles

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Last updated: 24 Dec 2024

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Abstract

This study explored callus initiation and somatic embryogenesis within GZ650 maize genotype, emphasizing the influence of distinct culture media and zinc oxide nanoparticles (ZnONPs). In this experiment, 800 immature embryos were cultured on four different media (control media and different concentrations of zinc media) (CM, ZM1, ZM2, and ZM3). Successful somatic embryogenesis was achieved, with ZM3 medium containing 4000 ppm ZnONPs demonstrating optimal callus induction. ZnONPs consistently outperformed the control in promoting embryogenic callus formation, notably with ZM3 resulting in 89.5% embryogenic calli compared to the control's (43%). Salinity treatments affect ZM3-derived embryogenic calli as well as callus performance. Genetic diversity analysis using inter-simple sequence repeats (ISSR) markers highlighted substantial variations and relationships among maize samples under salinity treatments. This research underscores the potential of ZnONPs to facilitate callus development, enhance plant tolerance against salinity, and assess genetic variation in response to ZnONPs and salinity stress.

DOI

10.21608/jacb.2024.291248.1084

Keywords

embryogenic callus, Plant regeneration, Zinc Oxide Nanoparticles, salinity stress, ISSR

Authors

First Name

A.

Last Name

Serag

MiddleName

M.

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Benha, Egypt.

Email

ahmed.serageldin@fagr.bu.edu.eg

City

-

Orcid

0000-0002-5051-3416

First Name

M.

Last Name

Basry

MiddleName

A.

Affiliation

Department of Plant Molecular Biology, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC),12619 Giza Egypt.

Email

-

City

-

Orcid

-

First Name

H.

Last Name

Zakaria

MiddleName

M.

Affiliation

Department of Plant Gene transfer, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), 12619 Giza Egypt

Email

-

City

-

Orcid

-

First Name

Fida

Last Name

Alosh

MiddleName

-

Affiliation

International Center for Agricultural Research in the Dry Areas (ICARDA), Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Refaat

MiddleName

H.

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Benha, Egypt.

Email

-

City

-

Orcid

-

First Name

T.

Last Name

El-Akkad

MiddleName

A.

Affiliation

Department of Genetics and Genetic Engineering, Faculty of Agriculture, Benha University, Benha, Egypt.

Email

-

City

-

Orcid

-

Volume

15

Article Issue

6

Related Issue

48763

Issue Date

2024-06-01

Receive Date

2024-08-04

Publish Date

2024-06-01

Page Start

75

Page End

85

Print ISSN

2090-3626

Online ISSN

2090-3707

Article File

JACB_Volume 15_Issue 6_Pages 75-85.pdf

PDF . 665.3kB

Link

https://jacb.journals.ekb.eg/article_371767.html

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https://jacb.journals.ekb.eg/service?article_code=371767

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

Enhancing Callus Initiation and Salinity Tolerance in Egyptian GZ650 Maize (Zea mays L.) Using Zinc Oxide Nanoparticles

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Type

Article

Created At

24 Dec 2024