This study was conducted at El- Gharbia Governorate to evaluate the quality of water for domestic purposes. The samples of water were collected from different sites during 2012 /2013 in order to determine and identify the bacterial isolates. In addition, certain physical and chemical parameters like temperature, pH, turbidity, electrical conductivity, total of some chemicals (hardness, partial hardness, calcium, magnesium, calcium hardness, magnesium hardness, SO₄, silica, iron, manganese, aluminum, phosphoras, ammonia), Nitrite, Nitrate and Chlorides were determined to evaluate quality of Nile, ground and tap water at five sites namely: Tanta, Elmehala-Elkobra, Mehalt Abo Ali, Samanod and Mehalt-Roh.  In a previous study at 2016 about 60 bacterial isolates were detected on selective media after isolation from the three sources of drinking water. These bacterial isolates were belonging to genera: Staphylococcus, Aeromonas, Proteus, Serratia and Pantoea.  In this study, the Vitek Microbial system was used for identified the bacterial genera speices as: Staphylococcus aureus, Aeromonas  hydrophila,   Proteus mirabilis, Serratia  plymythica and  Pantoea  agglomerans. The physical and chemical analyses showed that water qualities of three water sources are convenient for both aquatic life and domestic purposes as the normal range within a River Nile and tap water but in ground water are different.