The genomes of the six wheat genotypes under study; Masr1, Shandaweel1, Line1. Line2, Giza171 and Sakha94 were extracted. Ten IRAP primers were applied for detecting the rule of salinity stress in activation of retrotransposition rate in the studied bread wheat genotypes. The wheat genotypes were irrigated with 100 mM, 150 mM and 200 mM of NaCl or distilled water for control. IRAP technique developed all the ten markers in the wheat under the different levels of salinity. IRAP-2175 primer showed a number of new bands with molecular weights ranged from 400 bp to 550 bp giving a new retrotransposition locations due to the treatment with different concentrations of salinity. The second primer (IRAP-2198) revealed that bands with molecular sizes ranged from 470 bp to 1160 bp obtained a new retrotransposition appearance. The third IRAP marker was revealed by the IRAP-2197 primer with molecular sizes of 215 bp to 342 bp under all the three levels of salinity. The results revealed different patterns between control and treatments and the high levels of salinity led to new retrotransposition. This study revealed that PCR technique; like IRAP can reflect the activation of retrotransposition due to high salt levels. The obtained results were encouraging and it is better to use this technique due to their advantage; easy, fast, cheap and effectiveness.