Azotobacter chroococcum and Azotobacter vinelandii were isolated and purified from soil samples collected in El-Gharbia, (Qutor), Governorate. These Azotobacter isolates produced the hormones cytokinin, gibberellins and auxin. The current study was carried out to evaluate the impact of a potent Azotobacter strains on growth and productivity of wheat plants. Thus, after inoculation with Az. chroococcum, Az. vinelandii, and their mixture, the plant height, number of spikes/m², 1000-grain weight, grains yield, straw yield, total chlorophyll (mg g^-1), leaf nitrogen content, leaf area(cm²). Between the various treatments, there were noticeable differences in the total chlorophyll(mg g-1),leaf nitrogen content and leaf area(cm2). The different treatments (Azotobacter and concentrations of N) showed a substantial difference. The total chlorophyll (mg g-1),leaf nitrogen content and leaf area(cm²) were shown to considerably increase with an increase in N fertilization, reaching its maximum at an Azotobacter mixture of 75 kg/Fed.,protein content% The effects of nitrogen levels and Azotobacter types on wheat protein content are shown by the results in Table (8).The effects of the two Azotobacter strains (Az. vinelandii and Az. chroococcum) and the mixture on the protein content were 10.67%, 10.77%, and 10.85%, respectively, according to the data. The blend of Azotobacterand 75 kg N/fed had the highest protein content among the treatments on wheat crop, and several anatomical characteristics, including bundle size, leaf thickness, and size of xylem and phloem, increased as a result of the treatments.