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Genetic Diversity Among some Parental Varieties of Vicia faba L. Using ISSR and SCoT Molecular Marker Techniques

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Last updated: 24 Dec 2024

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Abstract

This study was executed to evaluate molecular variations among 13 genotypes of Vicia faba from Egypt and Kurdistan of Iraq for use in crop breeding programs in Kurdistan. The genetic relationships have been detected among these genotypes using ISSR and SCoT markers. Ten and twelve primers had successfully generated reproducible polymorphic amplicons that were suitable for studying the genetic variability between studied genotypes.  Out of 225 clear amplicons detected, (77 for ISSR and 92 for SCoT were polymorphic) with 70.71 and 70.03 polymorphism %, respectively. SCoT markers provided more discriminating data and were more informative with high UM%, DI, and Rp values (37.0, 0.23, and 3.81, respectively) compared with ISSR markers (25.7, 0.21, and 3.50, respectively). Cluster analysis and PCo analysis based on ISSR-SCoT combined data succeeded in the description of genetic diversity and heterogeneity within studied genotypes. Hence, setting the studied genotypes into four major groups. The first group included check variety only while the second group included sakha1, sakha3 and sakha4, the third group included only Giza843, and the last group contained all other genotypes, including all studied cultivars from Kurdistan. Crosses between divergent genotypes out of these groups may lead to the development of effective breeding strategies in the future. Therefore, we recommend more studies on the genetic improvement of these cultivars and their inclusion in breeding programs in Kurdistan of Iraq due to the significant genetic differences between them. Genetic Diversity Among some Parental Varieties of Vicia faba L. Using ISSR and SCoT Molecular Marker Techniques  

DOI

10.21608/jacb.2023.183614.1041

Keywords

Genetic diversity among of Vicia faba L, SCoT and ISSR

Authors

First Name

M.

Last Name

Albrifcany,

MiddleName

S.

Affiliation

Agronomist in Environment of Duhok government

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City

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Orcid

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First Name

Hajer

Last Name

Askander

MiddleName

S. A.

Affiliation

Field Crops department, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region of Iraq

Email

hajar.askandar@uod.ac

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Volume

13

Article Issue

12

Related Issue

38108

Issue Date

2022-12-01

Receive Date

2022-12-27

Publish Date

2022-12-01

Page Start

129

Page End

137

Print ISSN

2090-3626

Online ISSN

2090-3707

Article File

JACB_Volume 13_Issue 12_Pages 129-137.pdf

PDF . 812.8kB

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https://jacb.journals.ekb.eg/article_282574.html

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https://jacb.journals.ekb.eg/service?article_code=282574

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

Genetic Diversity Among some Parental Varieties of Vicia faba L. Using ISSR and SCoT Molecular Marker Techniques

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Article

Created At

24 Dec 2024