Surra is a chronic disease caused by Trypanosoma 
evansi which is transmitted mechanically by Tabanus and 
Stomoxys spp. In the present study, 300 blood samples were 
collected from six different animal species of cattle, buffaloes, 
sheep, goats, equines, and dogs from small farmer's holders, 
medium-sized farms, and from animals slaughtered at 
abattoirs in Alexandria and Beheira governorates. Blood 
samples were examined by Giemsa-stained blood smear and 
Polymerase Chain Reaction (PCR) to detect T. evansi. The 
study revealed that overall prevalence according to 
microscopy examination and PCR were 0.0% (0.0/300) and 
57.78% (104/180), respectively. The infection rates in 
different animal species were as follows; cattle, 46.67% 
(14/30); buffaloes, 70% (21/30); sheep, 40% (12/30); goats, 
30% (9/30); equines, 66.67% (20/30); dogs, 93.33% (28/30). 
The results showed that the highest infection rate in cattle, 
buffaloes, and sheep was recorded in Beheira, while in goats 
and equines, the highest infection rate was recorded in 
Alexandria. In dogs, the infection rate was the same in 
Alexandria and Beheira. Totally the highest infection rate 
(64.4%) was recorded in Beheira.