Tick-borne diseases (TBDs) are important factors that affect the development of animal husbandry worldwide, in both tropical and subtropical area. The aim of this study is to investigate the prevalence of the Piroplasma species in both Al Gabal al Akhder region in northeastern Libya and Ismailia, Egypt. Blood samples were collected randomly from 748 apparently healthy animals from various localities in Libya and Egypt during the period extending from January 2020 to January 2021. Giemsa-stained thin blood films were used for direct microscopy, conventional PCR was used for molecular identification of the samples. The results showed the prevalence of Piroplasma in Egypt was 23.9% in cattle and 19.5%in buffaloes, while in Libya the cattle was 27.6%, 31% in sheep, and 21% in goats. Real-time PCR in the diagnosis of blood parasites may give better results than conventional PCR.