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354208

In vitro MICROTUBERIZATION OF SOME POTATO (Solanum tuberosum L.) CULTIVARS AS RESPONSE TO MEDIA CONSTITUENTS AND PHOTOPERIOD

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Last updated: 24 Dec 2024

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Abstract

This work was carried out in cooperation with the plant production Dept., Fac. Technol. and Develop., Zagazig Univ., andGenetic Engineering and Tissue CultureLab., Genetics Dept., Fac. Agric., Kafr El- Sheikh Univ., during the period from 2020 to 2022 to study the performance of potato (Solanum tuberosum L.) genotypes, i.e. Diamant and Spunta for producing in vitro microtubers. The effect of media constituents, i.e. various combinations between kinetin and sucrose concentrations, as well as incubation photoperiod conditions were observed in two separate experiments. These experiments were arranged with Randomized complete design (RCD) with three replicates. As for the first experiment, there were fourteen treatments used, two potato cultivars with seven media combinations, i.e. 3, 6 and 9% sucrose without Kin or 6 and 9% sucrose either with 2 or 3 mgl-1 Kin. Meanwhile, in the other experiment, double nodes segment explants for both cultivars were cultured on MS medium (0.75% strength) contained 3 mgl-1 Kin + 6% sucrose and incubated under three photoperiods: 1) 16h/8h (light/dark), 2) 8h/16h (light/dark) and 3) full darkness (six treatments) two experiments were conducted in RCD with three replicates. The obtained results revealed that Diamant cultivar was more response to treatments than Spunta cv. MS medium supplemented with 3 mgl-1 Kin + 6 or 9% sucrose reduced the number of days to microtuber induction, increased tuberization percentage, number of microtubers after 30 and 75 days (at harvest) as well as average of microtubers fresh weight. Diamant cv. achieve the highest results for these parameters. Moreover, incubation cultures under photoperiod condition 8h/16h (light/ dark) or darkness provides best results as compared with light condition especially with Diamant cultivar. Conclusively,  it can be concluded that increasing Kin up to 3 mgl-1 + 6 or 9% sucrose with MS medium (0.75% strength) and incubated explants in short day or darkness particularly with  diamante cv. enhanced and improved the production and development of in vitro microtubers.  

Keywords

potato, tuberization, sucrose, kinetin, Photoperiod, microtubers

Authors

First Name

Amina

Last Name

Wafa

MiddleName

-

Affiliation

Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt

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City

Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt

Orcid

-

First Name

Wafaa

Last Name

Fekry

MiddleName

-

Affiliation

Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt.

Email

drwafaadel@hotmail.com

City

Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt.

Orcid

-

First Name

Aly

Last Name

Hassan

MiddleName

-

Affiliation

Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt.

Email

-

City

Plant Prod. Dept., Fac. Technol. and Dev., Zagazig Univ., Egypt.

Orcid

-

First Name

Ismail

Last Name

Khatab

MiddleName

-

Affiliation

Genetics Dept., Fac. Agric., Kafr El- Sheikh Univ., Egypt.

Email

-

City

Genetics Dept., Fac. Agric., Kafr El- Sheikh Univ., Egypt.

Orcid

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Volume

29

Article Issue

2

Related Issue

47544

Issue Date

2024-04-01

Receive Date

2024-03-12

Publish Date

2024-04-01

Page Start

81

Page End

98

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 29_Issue 2_Pages 81-98.pdf

PDF . 793.2kB

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https://jpd.journals.ekb.eg/article_354208.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=354208

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2

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

In vitro MICROTUBERIZATION OF SOME POTATO (Solanum tuberosum L.) CULTIVARS AS RESPONSE TO MEDIA CONSTITUENTS AND PHOTOPERIOD

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Article

Created At

24 Dec 2024