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342707

EFFECT OF NANO SELENIUM SUPPLEMENTATION ON SEMEN CHARACTERISTICS STORED AT COOLTEMPERATURE

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Last updated: 24 Dec 2024

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Abstract

Semen was collected once a week from four sexually mature buffalo bulls raised at International Livestock Management Training Center (ILMTC), Sakha, belonging to the Animal Production Research Institute, to evaluate the effect of addition different concentrations of selenium Nano-particles (SeNPs) on sperm motility, livability, abnormality, plasma membrane and acrosome integrity in semen stored at 5°C for different storage periods. Only ejaculates with mass motility of 70% or more were pooled for each collection day. The collected semen was diluted with Tris-extender and held in a water bath at 37°C, then divided into six portions. Different concentrations of SeNPs were added to the six diluted portions of semen (0 (control), 25, 0.5, 0.75, 1 and 1.25µg/mL /mLTris extender). Thereafter, all portion of semen were stored at 5°C (refrigerator temperature) for 0, 6, 24, 48, 72 and 96 h periods. Percentages of progressive motility, livability, abnormality, plasma membrane and acrosome integrity of spermatozoa were determined at various storage times. Results revealed that addition SeNPs improved all semen characteristics during different storage periods compared to control but the differences during the periods of 0, 24 and 48 h of storage were not significant.  While the differences were highly (P<0.05) significant among the extenders supplemented with SeNPs compared with control during the periods of 72 and 96 h of storage. Sperm motility, livability, plasma membrane and acrosome integrity decreased (P<0.05) by increasing storage period. However, sperm abnormality was increased affected by storage period,. Semen supplemented with 1.25 µg SeNPs was the highest sperm motility, livability, plasma membrane and acrosome integrity. Conclusively, the present results suggested the possibility of storage buffalo bulls semen at 5°C for 72 h with maintenance of sperm motility, livability, plasma membrane and acrosome integrity percentages above 40% in semen supplemented with 1.25µg/mL /mLSeNPs. Accordingly, it can be used for artificial insemination.  

Keywords

buffalo sperm, SeNPs, Storage, semen characteristics

Authors

First Name

Hassan

Last Name

El kholy

MiddleName

-

Affiliation

Biotechnology Research Department, Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Egypt.

Email

-

City

Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Egypt.

Orcid

-

First Name

El-shinawy

Last Name

El-Siefy

MiddleName

-

Affiliation

Biotechnology Research Department, Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Egypt.

Email

-

City

Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Egypt.

Orcid

-

First Name

Hassan

Last Name

El Kelawy

MiddleName

-

Affiliation

Department of Animal and Poultry Production, Faculty of Technology &amp; Development, Zagazig University, Zagazig, Egypt.

Email

drhassan_2105@yahoo.com

City

Faculty of Technology &amp; Development, Zagazig University, Zagazig, Egypt.

Orcid

-

First Name

Nagy

Last Name

El Gaafary

MiddleName

-

Affiliation

Department of Animal and Poultry Production, Faculty of Technology & Development, Zagazig University, Zagazig, Egypt.

Email

-

City

Faculty of Technology & Development, Zagazig University, Zagazig, Egypt.

Orcid

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Volume

28

Article Issue

4

Related Issue

41214

Issue Date

2023-10-01

Receive Date

2023-08-15

Publish Date

2023-10-01

Page Start

245

Page End

261

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 28_Issue 4_Pages 245-261.pdf

PDF . 682.7kB

Link

https://jpd.journals.ekb.eg/article_342707.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=342707

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4

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

EFFECT OF NANO SELENIUM SUPPLEMENTATION ON SEMEN CHARACTERISTICS STORED AT COOLTEMPERATURE

Details

Type

Article

Created At

24 Dec 2024