Background: Young adult men are most frequently diagnosed with testicular cancer. The age-standardized incidence rate for testicular cancer varies considerably by region. Aim: to evaluate the effect of health educational sessions regarding testicular cancer on awareness and practices of Port Said University male students. Subjects and method: Design: A quasi-experimental research design with (pre-test and post-test) was used. Setting: study was done at six faculties belonged to university of Port Said city, chosen randomly. Subjects: It was carried on 207 male students on Port Said university. Tools: two tools were used, tool I: Students' Testicular Cancer Awareness and Prevention Questionnaire, tool II: Testicular Self Examination reported Practice Sheet. Results: The majority of areas for improvement were the definition of the testicle (98.1%), the age at which testicular cancer is most likely to occur (90.8%), the signs to look for during a testicular self-examination (92.8%), and the significance of testicular self-examination (80.2%). Testicular self-examination showed improvement from pre to post sessions.  Conclusion: a marked improvement in the studied students' total score of awareness and practices levels regarding testicular cancer and TSE post-implementation of educational sessions. A statistical positive correlation was found between awareness and practices in post sessions. Recommendation: Continual health education for male university students that emphasizes improving their comprehension of TC and the value of TSE practice for the early diagnosis, in order to give kids this knowledge, health practitioners must also use communication platforms like social media.