Background: Mothers' knowledge and attitudes about hearing loss have a significant role in the early discovery and effective treatment of childhood hearing impairment. Aim: this study assess mothers' knowledge and attitude about early detection of hearing loss of their infants in Port Said. Subjects and Methods: A descriptive cross-section design. Setting: this study was conducted at pediatric clinics and immunization rooms in primary healthcare facilities affiliated to Egypt healthcare authority in Port Said. Subjects: Non-Probability, Purposive sampling of 279 mothers whom selected from above mentioned settings.  Tools: The data was collected using structured interviews and mothers' attitudes on infant hearing testing and early intervention. The Results: with a mean standard deviation of 32.22 7.49; 49.1% had overall fair knowledge of the causes and effects of hearing loss; 53.3% of respondents expressed a negative attitude toward the subject of hearing loss, whereas 70.6% recognized the value of early hearing loss detection. There was no statistically significant relationship between the mothers' opinions toward infant screening and early intervention for hearing loss and their knowledge of hearing loss screening. Conclusion: In general, more than half of the study's mom participants had a fair grasp of hearing loss, while roughly two thirds had a generally good attitude toward it. However, the mothers' knowledge of hearing loss was unrelated to their overall attitudes. Recommendations: The creation of public awareness campaigns must be taken into account in order to change parents' perceptions about and attitudes around infant hearing loss as well as neonatal screening and care.