Background: Patients in intensive care units frequently get infections. Adhering to infection control measures is the only method to safeguard healthcare personnel, patients, and the public against outbreaks acquired in hospitals. Aim of the   study was: to assess nurses' knowledge and practice regarding infection control measures. Determined human resources that affect the application of infection control measures. Subjects and Method: Design: A descriptive research design was utilized. Setting: In universal insurance hospitals at the port-said city. Subjects: The study's participants were all nurses(174 ) who worked in intensive care units. Tools: includes the Nurses' Knowledge Questionnaire, the Nurses' practice Checklist regarding the application of infection control, and the human resources that affect the application of infection control measures.  Results: In this study, (89.1%) of nurses had a satisfactory level of knowledge, (80.5%) of nurses had a satisfactory level of practice, and all ICUs participating in the study ( 100%) had a satisfactory level of infection control measures (70%) unsatisfactory human resources. Conclusion: Although the current study demonstrated a satisfactory level of knowledge, practice, and infection control measures for the application of infection control measures in intensive care units, the human resources are unsatisfactory. And statistical significant relation between nurses knowledge and practice. Recommendation: Plan for continuous education regarding infection control measures and updating knowledge and practice