Objectives: The aim of this study is to evaluate anti-bacterial effect of its and fissure sealants releasing fluoride treated by chlorhexidine. Patient and Methods: The study included thirty patients. Group A: Molars that have had pits and fissures sealed with fluoride-releasing materials. Molars in group B that have had pit and fissure sealants applied release fluoride and CHX. Isolation of the Tooth, Acid Etching, Sealant Placement and Curing, and Sealant Evaluation Following Sealant Placement are all part of the treatment protocols for the two groups. microbiological evaluation to determine the number of colonies of the S. mutant. Results: From baseline to one week, the mean percent reduction in streptococcus levels for group B was 34.06.6, while for group A it was 21.016.4. With a p value of 0.008, this was statistically significant and more prevalent in Group B. With a p value of 0.150, the mean percent drop in streptococcus levels from baseline to one month and three months was statistically insignificant. Conclusion: Small amounts of chlorhexidine added to sealants have minimal to no impact on their mechanical qualities while having no noticeable impact over the antibacterial activity of fluoride sealant. This can help the pediatric dentists to improve caries protection methods and provide a healthy mouth for children