Objectives: This study was conducted to evaluate the surface roughness, microhardness, stain removal effectiveness, and stainability of abrasive and non-abrasive toothpaste on the tooth enamel. subjects and methods: The sample were categorized into three groups; Group I; Enamel specimens without toothpaste brushing (control group), Group II; Enamel specimens with abrasive toothpaste brushing, Group III: Enamel specimens with non-abrasive toothpaste brushing. Results the use of abrasive and non-abrasive toothpastes resulted in significantly increase in surface roughness, and stainability, of enamel when compared to control group, while, the abrasive toothpaste resulted in significant higher surface roughness, stainability and stain removal when compared to the non-abrasive one. the surface microhardness showed that the use of abrasive and non-abrasive toothpastes resulted in significantly decrease in surface microhardness of enamel when compared to control group, while, the abrasive toothpaste resulted in higher significant decrease in surface microhardness when compared to the non-abrasive one. Conclusion: The use of abrasive and non-abrasive toothpastes can increase the surface roughness, decrease the surface hardness of enamel, increase the susceptibility of enamel surface to stains, can remove the stains. The use of abrasive toothpaste has higher harmful roughness effect on the enamel, decreases the enamel hardness, increases the susceptibility of enamel surface to stains, has the higher ability to remove the stains from the enamel surface.