Aim: This study was directed to evaluate the effect of chitosan, grape seed extract and chlorohexidine on resin dentin interface and nanoleakage evaluation of universal adhesives.
Materials and methods: A total of 60 upper premolars divided into 4 groups , with a total of 15 teeth in each group were used in this study. To study the effect of the three treatments groups with chitosan, grape seeds and chlorohexidine, ANOVA test was used for comparison of these materials with control group. After scrubbing and disinfection of every tooth by sodium hypochlorite and then distilled water, standardized Class V cavities were prepared, The 0.2% chitosan solution was prepared with 0.2 gram of chitosan. GSE was prepared with 6.5% conc. Chx with 2% concentration. All restorations were placed according to the manufacturer's instructions, using the incremental placement technique and were cured using the light-emitting diode, Scanning electron microscopy (SEMb)-SEM is well established technique to examine the interface between dentin restorative material, AgNo3 dye is used in this study ,as it's proved according to different studies that AgNo3 is suitable in photography.
Results: There was a significant difference in nanoleakage evalution between chx group and all remaining groups, with no significant difference in nanoleakge evaluation between chitosan group and Grape seed extract group. Nanoleakge increased with time. Conclusion: the application of CHX 2% before composite resin application is found to increase chance of nanoleakage in resin dentin interface.