ABSTRACT: The current study's target is directed to assessment of DNA ploidy on experimentally induced hamster buccal pouch carcinoma. Material and methods: five weeks old thirty male Syrian hamsters, weighing 80-120g. Three distinct groups of experimental animals were used (10 in each). GI (negative control): animals left untreated. GII (DMBA): animals were painted with 0.5 percent 7, 12-dimethylbenz (a) anthracene (DMBA) (3times/week/14 weeks). GIII (Paclitaxel- metformin): following DMBA application, animals injected intraperitoneally (IP) with paclitaxel (IP) (20mg/kg/ twice a week /3 weeks) and metformin (IP) (10 mg/kg/ 3 a week /3 weeks). After euthanization, all right pouches were surgically excised, bisected with one section fixed and processed for H&E and other section of the fresh tissue were used for flow cytometric (FCM) assessment. Results: Gross observation and histopathological changes were recorded. By FCM, the data exhibited that in GII DNA diploid was detected in (20%) of cases and DNA aneuploidy was detected in (80%) of cases while in GIII DNA diploid was detected in (51.3%) of cases. While DNA aneuploidy was detected in (48.7%) of cases. The SPF values of the diploid lesions in GII and GIII, ranged between (1.93% and 25.94%, 13.40%and 42.30%,) respectively. The SPF values of the aneuploid lesions in GII and GIII ranged between (10.25% and 51.50%, 5.50% and 42.50%,) respectively.
Conclusion: There is a substantial association between tumor DNA ploidy or SPF and responsiveness to metformin in combination with standard chemotherapeutic drugs.
Keywords: HBP carcinoma, Paclitaxel, DNA ploidy.