Apot experiment was carried outfor investigating the possibility of partial or entire replacement of mineral nitrogen fertilizers with organic and biofertilizers, aiming to improve the efficiency of nitrogen fertilization for barley plant grown on sandy calcareous soil collected from Agriculture Research Station in Nubariya.Mineral nitrogen fertilizer was added at the rates 30, 60, 90 and 120 mg N kg soil^-1.Phosphorus and potassium were added at the recommended rates of 15 mg P and 30 mg K kg soil^-1, respectively.Organic fertilizers were added from different sources "compost, chicken manure and pigeon dung" at a rate of 30 mg kg N soil^-1 based on their nitrogen content. Biofertilization was achieved through the addition of 5g pot^-1 serialin or/and 5g pot^-1 phosphorene; mixed thoroughly withsoil.Upon the observed data, thehighestdry matter yield was obtained when"compost + serialin" were applied to the soil in combination with mineral-N at addition level of "90 mg N kg soil^-1".Thehighest nitrogen uptake was obtained when the soil was treated with "chicken manure + serialin" combined with mineral-N at addition level of 60 mg N kgsoil^-1.Thehighest phosphorus uptake was obtained when soil treated with "chicken manure + phosphorine" combined with mineral-N at level of 60 mg N kg soil^-1. The highest potassium uptake was obtained when soil was treated with"chicken manure + serialin"combined with mineral-N at level of 90 mg N kg soil^-1.The highestyield of straw and grainsas well as protein contentwere obtained when soil was treated with"chicken manure + serialin" combined with mineral-N at level of 60 mg N kg soil^-1.