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317694

GENETIC POLYMORPHISM OF BUFFALO µ-CALPAIN GENE USING PCR-RFLP

Article

Last updated: 24 Dec 2024

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Tags

Biotechnology Research

Abstract

Calpain is a ubiquitous cytoplasmic cysteine protease, its activity is dependent on calcium. Two genes of calpain- CAPN1 (µ-calpain) and CAPN2 (m-calpain) have been identified. The micromolar calcium-activated neutral protease (CAPN1) gene encodes a cysteine protease, µ-calpain that degrades myofibril proteins under postmortem conditions and appears to be the primary enzyme in the postmortem tenderization process. Regulation of µ-calpain activity has correlated with variation in meat tenderness. Genetic polymorphism analysis of CAPN1 gene would likely aid in the development of selection criteria for improving meat tenderness. This study aimed to detect genetic polymorphism within intron 14 of buffalo CAPN1 gene using PCR-RFLP technique. Buffalo DNA was amplified using primers that were designed from the cattle CAPN1 gene sequence. The amplified fragments obtained from all tested buffalo DNA (100 animals) at 670-bp were digested with FokI endonuclease. All buffalo animals are genotyped as CC where all tested buffalo DNA amplified fragments were digested with FokI endonuclease and gave two digested fragments at 530-bp and 140-bp due to the presence of C base at position 4685 in all tested animals and the absence of T base in this position within intron 14.

DOI

10.21608/zjar.2010.317694

Keywords

Buffalo, µ-calpain gene, PCR, RFLP

Authors

First Name

Othman

Last Name

Othman

MiddleName

E.

Affiliation

Cell Biology Department, National Res. Center, Dokki, Egypt

Email

othmanmah@yahoo.com

City

-

Orcid

-

First Name

Fawzia

Last Name

Zayed

MiddleName

A.

Affiliation

Zoology Department, Fac. of Science, Zagazig Univ., Egypt

Email

-

City

-

Orcid

-

First Name

A. M.

Last Name

Abd El-Gawead

MiddleName

-

Affiliation

Zoology Department, Fac. of Science, Zagazig Univ., Egypt

Email

-

City

-

Orcid

-

First Name

M. R.

Last Name

Abd El-Rahman

MiddleName

-

Affiliation

Cell Biology Department, National Res. Center, Dokki, Egypt

Email

-

City

-

Orcid

-

Volume

37

Article Issue

3

Related Issue

43535

Issue Date

2010-05-01

Receive Date

2023-09-17

Publish Date

2010-05-01

Page Start

643

Page End

654

Print ISSN

1110-0338

Online ISSN

3009-7193

Article File

ZJAR_Volume 37_Issue 3_Pages 643-654.pdf

PDF . 408.1kB

Link

https://zjar.journals.ekb.eg/article_317694.html

Detail API

https://zjar.journals.ekb.eg/service?article_code=317694

Order

317,694

Type

Original Article

Type Code

842

Publication Type

Journal

Publication Title

Zagazig Journal of Agricultural Research

Publication Link

https://zjar.journals.ekb.eg/

MainTitle

GENETIC POLYMORPHISM OF BUFFALO µ-CALPAIN GENE USING PCR-RFLP

Details

Type

Article

Created At

24 Dec 2024