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297654

BIOLOGICAL ACTIVITIES OF Citrulluscolocynthis ETHANOLIC SEED EXTRACT

Article

Last updated: 24 Dec 2024

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Biotechnology Research

Abstract

The bitter gourd plant Citrulluscolocynthis (L.)  is a cucurbit that is widespread in desert areas. It has many pharmaceutical, medicinal, and nutritional uses due to its high content of biologically active compounds. In this study, the total phenolic compounds (TPCs) and total flavonoids (TFs) of ethanolicC. colocynthis seed extract were estimated. High-performance liquid chromatography (HPLC) was used to identify the bioactive phenolic compounds. On the other hand, the antioxidant and anticancer activities of this extract were evaluated. The extraction yield from
C. colocynthis seed using ethanol was recorded 10 g/100g seed. The total phenol (TPCs) content of the ethanolic extract obtained from C. colocynthis seed flour was 21 mg GAE g-1 dry extract, while the total flavonoid (TFCs) content was 15 mg QE g-1 dry extract. As well as the major phenolic and flavonoid compounds in C. colocynthis seed ethanolic extract, as determined by HPLC. Ellagic acid and cinnamic acid have the highest concentrations (894 and 746 µg/g dry extract, respectively) of all the components. The anticancer activity in vitro of the HCT116 and A-549 human cancer cell lines was evaluated using the MTT assay. The results of the MTT assay demonstrated that the C. colocynthis seed extract inhibited the proliferation of HCT116 and A-549 cells in a dose-dependent manner. C. colocynthis seed extract had an IC50 against HCT116 and A-549 (454, and 416 µg/mL, respectively). Therefore, C. colocynthis can be used as a source to produce pharmaceutical and medicinal products.

DOI

10.21608/zjar.2023.297654

Keywords

Bitter gourd, Total Phenolics, Total flavonoids, DPPH-assay, MTT-assay

Authors

First Name

Wafaa

Last Name

Mohamed

MiddleName

E.

Affiliation

Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

wafaaeid939@gmail.com

City

-

Orcid

-

First Name

R.A.

Last Name

Al-Masry

MiddleName

-

Affiliation

Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

-

City

-

Orcid

-

First Name

H.A.

Last Name

Elakkad

MiddleName

-

Affiliation

Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Osman

MiddleName

-

Affiliation

Biochem. Dept., Fac. Agric., Zagazig Univ., Egypt

Email

-

City

-

Orcid

-

Volume

50

Article Issue

2

Related Issue

41124

Issue Date

2023-03-01

Receive Date

2023-05-03

Publish Date

2023-03-01

Page Start

181

Page End

190

Print ISSN

1110-0338

Online ISSN

3009-7193

Article File

ZJAR_Volume 50_Issue 2_Pages 181-190.pdf

PDF . 505.2kB

Link

https://zjar.journals.ekb.eg/article_297654.html

Detail API

https://zjar.journals.ekb.eg/service?article_code=297654

Order

297,654

Type

Original Article

Type Code

842

Publication Type

Journal

Publication Title

Zagazig Journal of Agricultural Research

Publication Link

https://zjar.journals.ekb.eg/

MainTitle

BIOLOGICAL ACTIVITIES OF Citrulluscolocynthis ETHANOLIC SEED EXTRACT

Details

Type

Article

Created At

24 Dec 2024