This study was performed on six species belongs to genus Aloe grown in Egypt; namely; Aloe barbadensis, A. camperi, A. ciliaris, A. grandidentata, A. nobilis and A. vera. The aim of this study is to explore the taxonomic relationships between these species using randomly amplified polymorphic DNA (RAPD) markers to elucidate the pattern of genetic diversity among these individuals of different species of Aloe. In addition to the morphological characters. A total of 9 RAPD primers were screened of six spices of Aloe. The percentage of polymorphic bands was 91.33% with molecular size ranged from 0.100 kb to 2.5 kb. Only 3 of 29 bands were commonly detected in all samples which reflected certain homology of sample. From the obtained results the six Aloe species were divided into three clusters, cluster (I) containing Aloe barbadensis and Aloe vera at homology 20% related with cluster (II) at homology 7% which containing Aloe grandidentata and Aloe nobilis. The last cluster (III) containing Aloe camperi and Aloe ciliaris at homology 12%.