Two field experiments 2020-2021 and 2022-2023 were conducted to investigate the effect of foliar application with putrescine (0, 25, 50 ppm) and benzyladenine (0, 50, 100 ppm) on growth, yield parameters, seed oil percentage and fatty acids composition of chia (Salvia hispanica L.) plants grown under water stress conditions (40, 70% ETo and 100% ETo Full irrigation as control). The results showed that, both two water deficit irrigation treatments 70% and 40% ETo significantly decreased all studied growth and yield parameters of chia plants in both seasons as well as, these used water stress levels used decrease seed oil percentage and unsaturated fatty acids such as linolenic and Linoleic of chia plants compared with unstressed plants (control 100% ETo). In contrast, two water stress levels treatments induced a marked increase WUE and saturated fatty acids compared with unstressed plant (100% ETo). Date also indicated that the foliar application treatments of putrescine (at 25 ppm) and benzyladenine (at 50 ppm) alone or in combination with two water stress levels significantly increased all the above mentioned studied traits of growth, yield parameters and WUE in both season as well as seed oil percentage and unsaturated fatty acids composition in second season. Since, putrescine at 25 ppm was the most effective in this respect. Generally, the foliar application treatments with low concentration of putrescine and benzyladenine were the best for improving growth, productivity, seed oil percentage, and fatty acids composition of chia (Salvia hispanica L.) plants under normal or water stress conditions.