This study dealed with the prevalence of endoparasitic worms that infect the gastrointestinal tract of Spotted groupers (Epinephelus coioides) collected from Kuwait. Trematodes were found in Prosorhynchus pacificus with the incidence (8.3%), Prosorhynchus epinepheli with infection rate (11.6%) and in Prosorhynchus jexi with rate (6.6%). Nematodes were found in Hysterothylacium epinepheli with rate (5%) and in Cucullanus epinepheli with rate (10%). Acanthocephalan parasites Rhadinorhynchus sp. with rate of infection (8.3%). The total infection rate was 53.3 % of examined fish sample. P. epinepheli represent the highest prevalence percent while the rate of H. epinepheli represent the lowest rate of infection. The histopathological examination of the examined fish intestine revealed some pathological symptoms such as degeneration, necrosis and fibrosis in the site of infection.This study dealed with the prevalence of endoparasitic worms that infect the gastrointestinal tract of Spotted groupers (Epinephelus coioides) collected from Kuwait. Trematodes were found in Prosorhynchus pacificus with the incidence (8.3%), Prosorhynchus epinepheli with infection rate (11.6%) and in Prosorhynchus jexi with rate (6.6%). Nematodes were found in Hysterothylacium epinepheli with rate (5%) and in Cucullanus epinepheli with rate (10%). Acanthocephalan parasites Rhadinorhynchus sp. with rate of infection (8.3%). The total infection rate was 53.3 % of examined fish sample. P. epinepheli represent the highest prevalence percent while the rate of H. epinepheli represent the lowest rate of infection. The histopathological examination of the examined fish intestine revealed some pathological symptoms such as degeneration, necrosis and fibrosis in the site of infection.