Abstract
Investigating the prevalence of Staphylococcus aureus, particularly methicillin-resistant S. aureus (MRSA), in patients, healthcare workers (HCWs), and inanimate objects in the research area in Zagazig province was the main goal of the current study. S. aureus was analysed bacteriologically in samples taken from Zagazig University and El-Mabarrah Hospitals in Zagazig City, Sharkia Governorate, Egypt. The presence of nuclease (the nuc gene) was further verified in all S. aureus isolates using PCR. The presence of the mecA gene was then used by PCR to identify the MRSA isolates. 52 (16.5%) isolates of S. aureus were confirmed by the nuc gene amplification; of these, 14 (26.9%) were recognised as MRSA and 38 (12.1%) as non-MRSA isolates based on the nuc gene amplification. We found the percent of two groups which lower than forty five years old and higher than fifty five was (37.5%), female is (87.5%) more than male percent (12.5%).