The purpose of this work was to estimate the total phenolics and flavonoids content of alcoholic extracts of Gossypium barbadense L. parts such as bolls, flowers and leaves. The presence of several sorts of phenolics, flavonoids, tannins, carbohydrates, glycosides and saponins were shown by qualitative phytochemical screening of the methanolic extracts, whereas alkaloids, terpenoids, and steroids were lacking. The maximum total phenolics content (0.320±0.012 mg GAE/g) was determined in Gossypium barbadense L. leaves extract, whereas the highest total flavonoids content (0.488±0.024mg QE/g) was determined in its bolls extract. Since phenols and flavonoids are potentially active, we assessed the antimicrobial activities of methanolic extracts of Gossypium barbadense L. parts such as bolls, flowers and leaves. Methanolic extracts were examined for antibacterial activities against (Escherichia coli ATCC10536, Staphylococcus aureus ATCC6538, Salmonella enterica ATCC 14028, and pesudomonas aeruginosa ATCC 9027), whereas antifungal activities against two fungi (Aspergillus niger, Aspergillus fumigatus) using the disc diffusion method. The results were compared to the zone of inhibition caused by standard antibiotics that are commercially accessible. The results demonstrated that methanolic extracts of Gossypium barbadense L. parts such as bolls, flowers and leaves exhibit antibacterial activity against E.coli and S.aureus, whereas only the bolls and flowers extracts have action against P.aeruginosa. While the growth of S.enterica was inhibited by bolls extract only. On other hand, there are no anti-fungal properties against Aspergillus niger and Aspergillus fumigatus for all methanolic extracts of Gossypium barbadense L. parts.