Heavy metals are natural elements in the earth's crust. Some of these heavy metals are known as pollutants and are toxic, therefore, their amount in water should always be under control. One of this expulsion toxic elements is iron. As known microorganisms perform an important function in the bioremediation of contaminated water. Among of these microorganisms that are capable of bioremediation of heavy metals are A. oryzae. The aim of this study is to investigate the conditions for bioremediation of one of these heavy metals iron in water. Results showed that powder A. oryzae can be used for removal of iron from polluted water. As the dried A. oryzae in concentration 1g/100 ml was found to be more effective in the removal of iron from water at pH 3 with contact time 90 minutes. the A. oryzae successfully removed iron and has ability to be regenerated and reuse in the removal process.