Bio stimulants are gaining a more attention as being ecofriendly to human and environment. This study was conducted to evaluate the impact of two bio stimulants (Spirulina platensis and Trifolium alexandrinum) on some physiological parameters of one leguminous plant (Psium satvium L.).To achieve this goal four treatments were done in this experiment: Control without any treatment, Soil treated with 10 g of powdered Spirulina, Soil treated with 10 g of powdered Trifolium and finally soil treated with two bio stimulants (10 g Spirulina +10 g Trifolium).The obtained results clarified that all tested growth parameters were increased .Total pigments were increased with special references to combination of two bio stimulants. Parellel, Total carbohydrates were generally enhanced in all treatments if compared with control Water soluble vitamins as vitamin B Complex ,biotin, choline, folate, vitamin A,E and K were enhanced as a result of application of bio stimulants. On the other hand, No changes were revealed in Vitamin A, B2, B3, B5, B6, Cartenoid (α,B.Carotene, Cryptoxanthin, Leutin and zeaxanthin).