In this study was suspected E. coli were detected (9) pooled samples out of (120) examined pool samples with an incidence (7.5%); while (9) farms were positive for E. coli with an incidence of the farm infection of 47.3%.
The nine suspected E.Coli isolates were subjected to morphological and microbiological characterization of the colony (size, colour and shape), motility and gram reaction. All suspected colonies have pink colonies on Maconkey agar media, round, moist and raised.
The serogroup analysis nine (9) different E.Coli showed three different group were identified (2) O78, (3) O111 and (4) untyped group.
Nine isolates of E. coli were subjected to PCR. All isolates of E. coli were proved positive used this method of characterization and showed the specific expected PCR products at (720bp).
Performance parameter (body weight & body weight gain) at 28 days post infection in group 2 broilers showed lowest means body weight (1120 gm) in comparison of control group (1640 gm), while in groups 3, 4 and 5 body weight gain were showing significant increase (2060 gm), (1995 gm) and (1680 gm) respectively . feed consumption in control group (2673 gm) mean while in group 3, 4, 5 were (3069 gm), (3072 gm )(2973 gm) respectively.
Total protein showed high significant increase when compared with control and antibiotic groups.
Probiotic and prebiotic cause significant increase in body weight gain, feed consumption body weight and FCR.