The present study was conducted to evaluate the prevalence of Aeromonas spp. in Sharkia Governorate, Egypt. The samples comprised of tilapia (Oreochromis niloticus, n=160), mugil (Mugil cephalus, n=105), human stool (n=27) and fish sellers hand swabs (n=51). Fish samples were collected in sterilized polyethylene bags and transferred to the lab while,stool samples were collected from from Al Mabarrah, Al Ahrar and The University hospitals in Zagazig city, Sharkia Governorate, Egypt while, hand swabs were collected from fish sellers.A total of 15(4.3) Aeromonas spp. isolates were recovered and biochemically confirmed using biochemical test such as oxidase, triple sugar iron, indole production, Methyl Red, Voges-Proskauer, urease, of which, 4 were from tilapia viscera and muscles, 8 from mugil viscera and muscles and 3 from hand swabs. No isolates were recovered from stool samples. This study revealed that the prevelance of Aeromonas spp. In Mugil cephalus were more than in Oreochromis niloticus .